Download Maleate Hydratase. Identification of a Specific Maleate Hydratase in the Direct Hydrolysis Route of 2 the Gentisate Pathway 3 4. Kun Liua, b, Ying Xua, b, Ning-Yi Zhoua, b 5 6 7 8 9 Maleate hydratase, which hydrates maleate to form D-malate, was purified from a crude extract of Arthrobacter sp. Strain MCI2612 DEAE-Toyopearl, MALDI MATRIX, UNIVERSAL MALDI validation set PS, PMMA, PDMS, PEG and PSS Mp 5μ000-20μ000 certified according to DIN maleate hydratase. Bookcover of Maleate Hydratase. Omni badge Maleate Hydratase. Glycosyltransferase, Anthocyanidin, Flavonoid biosynthesis, Uridine diphosphate. Enzymes A-Z A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Diseases. Maleate hydratase. Name: Maleate hydratase (4.2.1.31). Structure & Reaction: 4.2. Maleate hydratase (malease) from Pseudomonas pseudoalcaligenes has been purified. The purified enzyme (98% pure) catalyzes the stereospecific addition of maleate and pyruvate, without prior cis, trans isomerization, using a GSH-independent maleylpyruvate hydratase and maleate isomerase were not detected. Identification of a Specific Maleate Hydratase in the Direct Hydrolysis Route of the Gentisate Pathway.Appl Environ Microbiol.2015. Rhea-Reaction-ID, 23695 23694 23692 23693. MetaCyc-Reaction-ID, MALEATE-HYDRATASE-RXN View all entries for reaction D-Malate <-> H2O + Maleate maleate hydratase,maleate hydratase,maleate hydratase, COG1024 Enoyl-CoA hydratase/carnithine racemase; Belongs to the enoyl-CoA Asp/Glu/Hydantoin racemase family protein; COG3473 Maleate cis-trans Maleate hydratase activity was measured from the decrease in the absorption at 240 nm. The reaction mixtures (final volume, 0.5 ml) contained |__(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity |__lupan-3beta,20-diol synthase activity |__maleate hydratase activity |__mannonate (3R)-hydroxymyristoyl-[acyl-carrier protein] dehydratase activity 16-alpha-hydroxyprogesterone dehydratase activity maleate hydratase activity. Maleate hydratase, which hydrates maleate to form D-malate, was purified from a crude The maleate hydratase appeared as two bands corresponding to. The maleate is then hydrated to yield D-malate (Hopper. Chapman, and Dagley maleate and citramalate hydratases were also present. These results would. Summary: 1) The partial enrichment of a new enzyme, di- methylmaleate hydratase from Clostridiwn bar- keri and some of its characteristics are described. 1, MALEATE CIS TRANS ISOMERASE, Author keyword, 11, 100%, 2%, 6. 2, L MALIC ACID 5, MALEATE HYDRATASE, Author keyword, 3, 100%, 1%, 3. Purification and characterization of maleate hydratase from Arthrobacter sp. Strain MCI2612 [1993]. Ueda, M. (Mitsubishi Kasei Corp., Yokohama (Japan)) Asano Enzyme Commission Primary Name: maleate hydratase. Enzyme Commission Synonyms: D-malate hydro-lyase, malease, (R)-malate hydro-lyase. Standard (3) maleylpyruvate hydrolase; (3) fumarylpyruvate hydrolase, (3) maleate hydratase; (3) fumarase 2-Hydroxymuconic semialdehyde is also a metabolite in the Hydratase enzymes are biocatalysts that are capable of turning one form of deficient alkenes, such as maleate, or the CoA thioesters of carboxylic acids. D -Malate can be produced in several ways, but the approach using the lyase maleate hydratase (malease; EC 4.2.1.31), which catalyzes the hydration of
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